Download e-book for iPad: Applications of Monolithic Column and Isotope Dimethylation by Fangjun Wang

By Fangjun Wang

ISBN-10: 3642420079

ISBN-13: 9783642420078

ISBN-10: 3662524325

ISBN-13: 9783662524329

In this thesis, the writer outlines the advance of recent monolithic columns and isotope dimethyl labeling recommendations and their purposes in high-performance proteome analyses. even though kinds of monolithic columns were greatly constructed for chromatography and electrophoresis separation, their software in proteomics for complicated peptide combinations separation remains to be a problem. the writer discusses the practise of recent monolithic columns and optimization of chromatography separation power to enhance assurance and accuracy of proteome research. additional, the writer describes a unique on-line multidimensional chromatography procedure mixed with computerized on-line isotope labeling, which considerably improves the throughput, sensitivity and accuracy of quantitative proteomics. as well as the advance of recent applied sciences, the writer investigates the proteome and phosphoproteome expression alterations of scientific hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer’s illness. The paintings during this thesis has ended in a number of courses in high-profile journals within the fields of analytical chemistry and proteome research.

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Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis (Springer Theses) by Fangjun Wang

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